PCR Fundamentals - 384-016-19

PCR Fundamentals

by Melissa Reske, BA and Catherine J. Swift, MT(ASCP)
(4.6 / 186 ratings )

This course is credentialed for:
Laboratory (1.00 Contact Hr)

Price: $10.00
CE-PRO Price: $5.00


The polymerase chain reaction is arguably one of the most common and useful molecular experimental techniques employed in diagnostic laboratories today. In the laboratory, the uses for PCR include disease and infection diagnosis, mutation detection, pathogen or pathogenic response detection, template amplification for downstream applications such as sequencing, and various other uses. This module serves as a review or introduction to the basics of polymerase chain reaction technology. Knowledge of a reaction's components and steps can aid a laboratory technologist in improving both troubleshooting and interpretation skills, both of which are important abilities to have and be constantly updating in the laboratory.


The goal of this program is to give the laboratory professional an overview and review of polymerase chain reaction and its uses in the laboratory as well as to provide the technologist with helpful tools for troubleshooting when reactions do not proceed as planned. After studying the information presented here, you will be able to:
  • Identify the basic components of the polymerase chain reaction and their functions within the reaction itself
  • Describe what is occurring during each of the phases of a polymerase chain reaction
  • Identify the main difference(s) between a basic polymerase chain reaction and a real-time polymerase chain reaction
  • Identify uses for the polymerase chain reaction in a diagnostic laboratory setting
Level of Instruction: Intermediate

Approval Information
This course is intended for laboratory professionals.
Relias LLC is approved as a provider of continuing education programs in the clinical laboratory sciences by the American Society for Clinical Laboratory Science (ASCLS) Professional Acknowledgment for Continuing Education (P.A.C.E.®) Program (provider #384).
384-016-19 "PCR Fundamentals" is approved 02/27/2019 to 02/28/2021.
OnCourse Learning is also approved by the Florida Board of Clinical Laboratory Personnel (provider # 50-1489).

Course Originally Released on: 12/5/2017
Date of Most Recent Review: 12/5/2017
Course Termination/Update Date: 2/28/2021

Unless stated above, the planners and authors of this course have declared no relevant conflicts of interest that relate to this educational activity. Relias LLC guarantees this educational activity is free from bias.

Please see CE Course Instructions to learn how to earn CE credit for this module.


For Florida professionals:

This course completion will be electronically reported to CE Broker as required per Florida Statute 456.025(7). Click here to view your CE Broker transcript and check the status of your CE requirements with a FREE 7-day CE Broker trial subscription. It may take up to 24 hours for the course to appear on your CE Broker transcript.

It is now required that all CE is completed and reported to CE Broker before you renew your license. When you complete continuing education with us, OnCourse Learning will report your hours for you.

Read more about the new license renewal process at AreYouRenewalReady.com and claim your free Basic Account in CE Broker to see what has already been reported for you.

Please check the licenses/certifications section under my account (after logging in) to make sure you have entered a valid FL license number. This information is required for correct reporting of your course completions to CE Broker.

Reports to CE Broker

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Lateeshia T - Owings Mills, MD  ·  Jun 01, 2020
Detailed information
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Joseph W - Concord, CA  ·  May 28, 2020
ell your friends and colleagues about this course. Your answers will help thousands of healthcare professions make a better decision.
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Very Informative and very interesting
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Derek S - Ball, LA  ·  May 09, 2020
Very informative. I thoroughly enjoyed the content.
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Dexter D - Williston, ND  ·  Apr 14, 2020
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Price: $10.00
CE-PRO Price: $5.00